Composite
pc-am

Part:BBa_K2967005:Design

Designed by: Rui Wang   Group: iGEM19_NEU_CHINA   (2019-07-17)


a biosignal amplifier


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 957
    Illegal XbaI site found at 2233
    Illegal SpeI site found at 980
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 980
    Illegal NotI site found at 1911
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1892
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 957
    Illegal XbaI site found at 2233
    Illegal SpeI site found at 980
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 957
    Illegal XbaI site found at 2233
    Illegal SpeI site found at 980
    Illegal NgoMIV site found at 534
    Illegal AgeI site found at 121
    Illegal AgeI site found at 127
    Illegal AgeI site found at 175
    Illegal AgeI site found at 741
    Illegal AgeI site found at 766
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1102
    Illegal SapI.rc site found at 1735


Design Notes

we use T7 termonator to ensure the termination on the plasmid and we optimize the sequence of the rbs to improve the amplifier.


Source

it is based on the pathway in the hrp (hypersensitive response and pathogenicity) gene regulatory network for Type III secretion in Pseudomonas syringae.

References