Composite
pc-am
Part:BBa_K2967005:Design
Designed by: Rui Wang Group: iGEM19_NEU_CHINA (2019-07-17)
a biosignal amplifier
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 957
Illegal XbaI site found at 2233
Illegal SpeI site found at 980 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 980
Illegal NotI site found at 1911 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1892
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 957
Illegal XbaI site found at 2233
Illegal SpeI site found at 980 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 957
Illegal XbaI site found at 2233
Illegal SpeI site found at 980
Illegal NgoMIV site found at 534
Illegal AgeI site found at 121
Illegal AgeI site found at 127
Illegal AgeI site found at 175
Illegal AgeI site found at 741
Illegal AgeI site found at 766 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1102
Illegal SapI.rc site found at 1735
Design Notes
we use T7 termonator to ensure the termination on the plasmid and we optimize the sequence of the rbs to improve the amplifier.
Source
it is based on the pathway in the hrp (hypersensitive response and pathogenicity) gene regulatory network for Type III secretion in Pseudomonas syringae.